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title How to choose antibodies

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Provider: Shanghai Jinma Experimental Equipment Co., Ltd Release time: July 29, 2020 reading times: 24 times >>Enter the company's stand
Immunoglobulin is a large Y-shaped protein secreted by plasma cells (effector B cells) and used by the immune system to identify and neutralize foreign substances such as bacteria and viruses. It is found only in the blood and other body fluids of vertebrates and on the cell membrane surface of B cells. Antibodies recognize a unique feature of a specific foreign object, which is called an antigen.
1. Selection of primary antibody
(1) Determine the name of antibody, pay attention to the Chinese and English name, alias, subtype and other information.
(2) The species, human, mouse, rat, etc. were determined. Our antibody instructions all list the applicable species that have been verified by the antibody. You can select the antibody suitable for your experiment according to the species verified in the manual.
(3) Determine your type of experiment, ELISA, WB, IHC, ICC, or FACS. Each antibody specification of biorbyt will list the applicable types of the antibody that have been verified. If the application type is not mentioned in the antibody specification, it does not mean that the antibody is not suitable for this type of analytical application, but only that it has not been verified by such experiments.
(4) Single polyclonal antibody selection. In general, monoclonal antibodies have strong specificity, but the affinity is relatively small, and the sensitivity of detection antigen is relatively low; while the specificity of polyclonal antibodies is slightly weak, but the antibodies have strong affinity and high sensitivity, but they are prone to non-specific staining (which can be avoided by blocking, etc.).
(5) The structural properties of the sample antigen protein. Understanding the structure and properties of the sample antigen protein is helpful to select the most appropriate antibody. The structural domain of the sample protein to be tested and whether the sample will be denatured during extraction and processing, and the change of protein spatial conformation will affect the immune affinity reaction of antibody.
2. Selection of secondary antibody
(1) Source of species and genera. The source of the second antibody is mainly determined according to the origin of the first antibody. If the first antibody is from mice, the second antibody can be purchased from the mouse (sheep, rabbit, etc.).
(2) The selection of markers. There were HRP, biotin, fluorescein and other markers. The second antibody of WB was mainly HRP and biotin labeled, while immunofluorescence staining could select different fluorescein labeled secondary antibodies, such as FITC, Cy3, PE, etc.

The company's main business focuses on reagent preparation and animal experiment services.
It mainly provides detection reagents for life science research, and the antibody center has a complete platform for preparation of polyclonal antibodies and monoclonal antibodies. The preparation center is responsible for the development of detection kits, mainly ELISA kits. The testing center is responsible for the detection and quality control of all protein, antibody and test kit products, and implements three-level quality control, including raw material testing, semi-finished product testing and finished product testing.

key word: How to choose antibodies ??

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