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Your location: home page ?>? Product exhibition hall ?>? Chemical raw materials ?>? Chemical Reagents ?>? Biochemical reagent Rat vascular endothelial growth factor A (VEGF-A) kit

 Rat vascular endothelial growth factor A (VEGF-A) kit

Rat vascular endothelial growth factor A (VEGF-A) kit

  • Price: 4800 yuan
  • model:
  • Place of production: Mainland China
  • Visit: 0 times
  • Release date: August 14, 2020 (Update Date: August 14, 2020)

Shanghai Jinma Experimental Equipment Co., Ltd two year

  • Telephone: thirteen billion two hundred and sixty-two million two hundred and sixty-five thousand eight hundred and fifty-four
  • mobile phone: thirteen billion six hundred and thirty-six million three hundred and fifty-one thousand and seventy-three
  • Contact person: Manager Li( When contacting me, please explain that it was seen in Baichuan. Thank you!
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The imported rat vascular endothelial growth factor A (VEGF-A) ELISA kit was used for quantitative detection of serum, plasma, tissue, cell supernatant and related liquid samples in vitro;
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[product literature support]
Influence factor (if) = 5.5: anti inflammation effects of corn silk in a rat model of carrageenin induced pleurisy(
http://caijubei.com/c153249/down/d4286.html

Product Name: rat vascular endothelial growth factor A (VEGF-A) ELISA Kit
Cooperation unit: University / hospital / Scientific Research Institute;
The experiment type: sandwich method / indirect method / competition method;
Detection range: 5 pg / 400 ml
Sensitivity: the detection concentration of best was lower than 1.0 μ g / ml.
Specificity: no cross reaction with other soluble structural analogues.
Repeatability: the coefficient of variation within the plate is less than 10%, and the coefficient of variation between the plates is less than 15%.
Scope of application: for in vitro quantitative detection of serum, plasma, tissue, cell supernatant and related liquid samples;
Storage conditions: 2-8 ℃;
Solemnly declare: This product is only for scientific research and experiment, not suitable for clinical diagnosis!
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Antibody detection series: streptolysin O antibody ELISA kit, AQP AB kit, HINI kit;
Special protein detection: nearly ten species of albumin, globulin ELISA kit.
explain
1. Due to the existing conditions and the level of science and technology can not fully identify and analyze all raw materials provided by all suppliers, this product may have certain quality and technical risks.
2. The results of the experiment are closely related to the effectiveness of the reagent, the relevant operation of the experimenter and the experimental environment at that time. Please make sure to prepare sufficient specimen backup.
3. Different batches of the same product may have some differences, such as detection limit, sensitivity and color development time. Please carry out the experiment according to the instructions in the kit. The electronic version of the manual on the website is only for reference.
4. Only when all the matching reagents of this kit are used can the detection effect be guaranteed, and the products of other manufacturers cannot be mixed. Only by strictly following the experimental instructions of this kit can we get the best detection results.

matters needing attention
1. Carry out temperature cultivation in strict accordance with the specified time and temperature to ensure accurate results. All reagents must reach room temperature 20-25 ℃ before use. Refrigerate the reagent immediately after use.
2. Incorrect plate washing can lead to inaccurate results. Before adding the substrate, make sure that the liquid in the hole is dried as much as possible. Do not let the micropores dry out in the process of warm cultivation.
3. Eliminate the residual liquid and finger print at the bottom of the plate, otherwise the OD value will be affected.
4. The substrate solution should be colorless or light color, and the substrate solution that has turned blue cannot be used.
5. Avoid cross contamination of reagents and samples to avoid wrong results.
6. Avoid direct exposure to strong light during storage and incubation.
7. After balancing to room temperature, open the sealing bag to prevent water droplets from condensing on the cold strip.
8. Any reaction reagent should not contact with bleaching solvent or strong gas emitted by bleaching solvent. Any bleaching component will destroy the biological activity of the reagent in the kit.
9. Do not use expired products.
10. If the disease is likely to spread, all samples should be well managed and the samples and detection devices should be handled according to the specified procedures.

Reagent preparation
The kit should be taken out from the refrigerated environment and should be balanced at room temperature before use.
Dilution of 20 × washing buffer: dilute distilled water by 1:20, that is, 1 part of 20 × washing buffer solution plus 19 parts of distilled water.

Operation procedure of rat vascular endothelial growth factor A (VEGF-A) ELISA Kit
1. Take out the required strips from the aluminum foil bags after 20 minutes of room temperature balance, and put the remaining strips back to 4 ℃ with self sealing bags.
2. Set up the standard sample hole and sample hole, and add 50 μ l standard substance of different concentration into the standard sample hole;
3. Add 50 μ l sample into the sample hole; do not add the blank hole.
4. In addition to the blank hole, 100 μ l HRP labeled antibody was added into each well of standard sample hole and sample hole. The reaction hole was sealed with sealing plate membrane, and incubated in 37 ℃ water bath or incubator for 60 min.
5. Discard the liquid, pat dry on the absorbent paper, fill each hole with washing solution (350 μ L), let stand for 1 min, throw away the washing liquid, pat dry on the absorbent paper, and wash the plate for 5 times in this way (also use washing machine to wash the plate).
6. 50 μ l substrate a and 50 μ L B were added into each well and incubated for 15 min at 37 ℃.
7. Add 50 μ l termination solution into each well, and within 15 minutes, determine the OD value of each pore at 450 nm.
Shanghai Jinma Experimental Equipment Co., Ltd. was established in 2010, focusing on life science and biomedical field for many years. It is a new high-tech company integrating R & D, production and sales. Our company provides a variety of biological detection kits for universities, scientific research institutions, hospitals, pharmaceutical and diagnostic reagent manufacturers and other customers, such as enzyme-linked immunosorbent assay, enzyme-linked immunosorbent assay kit, tszelisa kit, original ELISA kit, ELISA test kit, import of conventional biochemical reagents, antibodies, chemical reagents, Pharmacopoeia grade reagents, standards, serum and other scientific research Our products cover the fields of molecular biology, biochemistry, cell biology, immunology, proteomics, biopharmaceutical and diagnostic reagents. After many years of development, we have obtained many brands, such as ambcr, HbCO, HbCO, etc. Since its establishment, the company has attached great importance to the construction of enterprise informatization. We have adopted the advanced internal supply chain information processing platform to ensure the accurate and efficient processing of customer orders. Our customer relationship management system and customer relationship management team can respond to customers' pre-sale and after-sales demands in the first time, and pay attention to customer experience and satisfaction. We have hundreds of professional customer service engineers to provide you with professional and patient service. We are professional, so trustworthy; we focus, so we develop rapidly; we believe in "customer first", we adhere to "integrity innovation, win-win cooperation"; we will do our best to provide you with convenient and professional procurement services, save your time and cost. We firmly believe that through our unremitting efforts, Shanghai Jinma Experimental Equipment Co., Ltd. will become a "professional supplier with professional, large-scale, complete varieties and complete supporting services in the field of life science and chemistry", provide users with "one-stop" service in the field of biochemical science, and become the leader of this industry.
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