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Hengyuan Literature: application of IL-37, TNF-a rat ELISA kit in literature

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Brief introduction

The role and significance of interleukin-37 and its related factors in rat model of acute lung injury
Sun Yunhui, Wang Yixin, Ma Xuemei, Yang Xiaodong, Bao Wenhua
Department of respiratory medicine, the First Affiliated Hospital of Jiamusi University (Heilongjiang Jiamusi 154003)

[Abstract] Objective To study the expression of interleukin-37 (IL-37) and related factors in lung tissue of rats with acute lung injury (ALI), and to elucidate the role and significance of IL-37 in acute lung injury, and further explore the pathogenesis of acute lung injury Forty five clean grade Wistar rats were randomly divided into three groups: healthy control group, bleomycin group and dexamethasone treatment group, with 15 rats in each group. Bleomycin group and dexamethasone treatment group were given bleomycin 4mg / kg to make acute lung injury model The rats in the dexamethasone treatment group were intraperitoneally injected with dexamethasone 3 mg / kg every day on the second day of acute lung injury modeling. The rats in the healthy control group and bleomycin group were intraperitoneally injected with normal saline as the control. The rats in each group were killed on the 7th, 14th and 28th days after modeling. The pathological changes of lung tissue were evaluated by HE staining. The contents of IL-37 and serum tumor necrosis factor-n (tnf-n) in lung tissue homogenate were measured, respectively Results histopathological examination showed that the structure of lung tissue in healthy control group was complete, and there was no inflammatory and fibrosis change; the lung tissue of bleomycin group and dexamethasone treatment group developed from early acute alveolar inflammatory change to late stage The changes of acute inflammation and pulmonary fibrosis in dexamethasone group were less than those in bleomycin group at the same time point. The levels of IL-37, TNF-a and IL-18 mRNA in bleomycin group and dexamethasone treatment group increased to the highest point on the 7th day, then decreased gradually, and then increased to the 28th day. The differences were statistically significant (I > all < 0.05) It may be related to the regulation of IL-18 and tnf-n transduction.
[Key words] acute lung injury, interleukin-37, interleukin-18, tumor necrosis factor-A, acute lung injury (ALI) is a progressive aggravation caused by severe infection, injury, shock and other factors, characterized by hypoxemia Acute respiratory insufficiency or respiratory failure. The pathogenesis of Ali has not been fully elucidated. The existing research results suggest that the excessive imbalance of inflammatory response is one of the most important factors leading to the occurrence and development of Ali. Interleukin-37 (IL-37) is a cytokine of IL-1 family. At present, it is believed that IL-37 can regulate innate and innate immune response through negative feedback regulation mechanism, inhibit the production of proinflammatory cytokines and chemokines, and regulate gene transcription However, excessive inflammatory response was inhibited [31. Previous studies have shown that IL-37 plays an important role in inflammatory and immune diseases such as rheumatoid arthritis and ulcerative colitis by regulating IL-18 and tumor necrosis factor-A (TNF-a). In order to clarify the role and significance of IL-37 and related factors in the pathogenesis of Ali, IL-37 level, IL-18 mRNA expression and serum TNF-a level were observed in ALI rats.
1. Materials and methods
1.1 materials
Bleomycin hydrochloride for injection (Haizheng Pfizer Pharmaceutical Co., Ltd.), hematoxylin eosin (he) staining reagent (Hubei Jinyuan Medical Technology Co., Ltd.); nikoneclipse50i microscope (Beijing Zhongyi Optical Technology Development Co., Ltd.) IL-37 TNF-a rat ELISA Kit (Shanghai Hengyuan Biotechnology Co., Ltd.) , RNA Extraction Kit (Beijing Adelaide Biotechnology Co., Ltd.), cDNA reverse transcription kit and PCR kit (Dalian Bao Bioengineering Co., Ltd.).


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